Authors:

Abstract

Cobalt (Co²⁺) is an essential constituent in the human body while excessive exposure leads to severe systemic toxic reactions which highlight the importance of developing effective methods to detect Co²⁺ ions. A simple and highly efficient fluorescence enhanced turn OFF-ON chemosensor was synthesized to detect the paramagnetic Co²⁺. The ligand, N-((1H-indol-3-yl) (phenyl)methyl)aniline (L), was synthesized in 92% yield by means of hydrated ferric chloride catalyzed one -pot multicomponent microwave irradiation in the presence of Indole, benzaldehyde, and aniline as reactants. The major green principles of waste prevention, high atom economy (94.3%), green solvent, higher energy efficiency, and catalysis were the highlights of the ligand synthesis. The ligand exhibited remarkable fluorescence enhancement with Co²⁺ and a turn ON ratio of over 160-fold in MeOH/H₂O (at pH 3.5) solution at an excitation wavelength of 369 nm in the Ultra-Violet range. The detection limit of L- Co²⁺ was 2.2 μM. The excitation and the emission spectra indicated stoke’s shift of 93 nm which supports the fluorescence enhancement observed in L- Co²+ with respect to the free ligand. The Job’s plot indicated fluorometric sensing of Co²⁺ ascribed to the complex formation with a stoichiometric ratio of 2:1 (L-Co²⁺). Furthermore, the high linearity (r² =0.992) observed in the Benesi Hildebrand plot in a wide concentration range of 0.5−80 μM confirmed the above stoichiometric ratio. The association constant (Ka) for the L-Co²⁺ was determined to be 8.382 ×1 0⁴ M⁻¹ ± 5.8 ×103M⁻¹.The prepared Co²⁺ fluorometric probe indicated long-term stability in −18℃ up to 45 days. Furthermore, the presence of Fe²⁺ and Fe³⁺ in the medium with Co²⁺ exhibited an interference effect in the fluorescence intensities. Upon further concentration studies, it was evident that the interference of Fe²⁺ and Fe³⁺ starts around 10.00 μM and rises exponentially.

Keywords: MCR, Green synthesis, Fluorescent Chemo-sensor, Turn OFF-ON, Cobalt (II), Indole derivatives

How to Cite: Azees, R., Cooray, A.T. and Kumarasinghe, K.G.U.R., 2021. Microwave-assisted one-pot multicomponent synthesis of indole derived fluorometric probe for detection of Co²⁺ ions. Vidyodaya Journal of Science, 24(2), pp.27–42.